Figure 6 | Cell Death & Differentiation

Figure 6

From: Necroptosis in microglia contributes to neuroinflammation and retinal degeneration through TLR4 activation

Figure 6

Anti-inflammatory and neuroprotective effect of Nec-1 in NMDA-induced neural injury. (a and b) Western blot showed that Nec-1 successfully downregulated necroptosis kinases (RIP1 and RIP3), as well as inflammatory cytokines (TNF-α, CCL2 and IL-17). n=4 retinae per group. (c) Nec-1 treatment reduced the number of apoptotic neuronal cells (green) in the GCL and INL after NMDA injury measured by TUNEL assay. (d) H&E staining showed that more retinal ganglion cells remained after Nec-1 injection. n=6 eyes and six microscope fields per eye. Scale bar: 100 μm. *P<0.05; **P<0.01; ***P<0.001

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