Figure 7
SLY protein interacts with TBL1XR1 protein. (a) SLY and TBL1XR1 antibody detection of WT and Sly-deficient (Sly-KD) whole-testicular extracts (INPUT) and of corresponding extracts immunoprecipitated with SLY antibody (IP:SLY). SLY immunoprecipitation performed on Sly-KD testicular extracts and immunoprecipitation with purified rabbit IgG on WT extracts (IP:IgG) were used as negative controls. (b) SLY and TBL1XR1 antibody detection of whole-testicular extracts from WT and FLAG-SLY mice (INPUT) and of corresponding extracts immunoprecipitated with FLAG antibody (IP:FLAG). FLAG immunoprecipitation performed on WT testicular extracts was used as a negative control. (c) SLY and TBL1XR1 antibody detection of WT whole-testicular extracts (INPUT) and immunoprecipitated with TBL1XR1 antibody (IP:TBL1XR1). TBL1XR1 immunoprecipitation performed on Sly-KD testicular extracts and immunoprecipitation with purified rabbit IgG on WT extracts (IP:IgG) were used as negative controls. Arrows indicate the specific band (at expected sizes of 38kDa for SLY and 64kDa for TBL1XR1) and stars, non-specific bands (which disappear in negative controls)
