Figure 2 | Cell Death & Differentiation

Figure 2

From: Nucleosome eviction along with H3K9ac deposition enhances Sox2 binding during human neuroectodermal commitment

Figure 2

Nucleosome remodeling upon the differentiation. (a) Nucleosome fuzziness is significantly decreased in hNECs. (P-value=2.2e−16, Student’s t-test). (b) Composite nucleosome distribution around TSS. (c) NDR formation in hNECs around TSS of a set of genes. Representative neuroectodermal genes are listed in the middle whose expression change folds are indicated at right. Arrows indicate upregulation in hNECs. (d) ChIP-qPCR confirmation of decreased nucleosome occupancy in NDRs of the selected neuroectodermal genes. Error bars represent SEM. (*P<0.05, **P<0.01, Student’s t-test). (e) GSEA showing that nervous system development genes are enriched in the genes in c with an NDR in their promoters and upregulated in hNECs. (f) Nucleosomes evicted in hNECs are abundant with H2A.Z nucleosomes. (g) NDRs remain around TSS of pluripotency genes whose expression levels are greatly decreased in hNECs. (h) Track view of NDRs around TSS of pluripotency TF Nanog maintained in both hESCs and hNECs. Green box indicates the NDRs

Back to article page