Figure 6

ZHX2 represses Notch1 promoter activity via NF-YA. (a) HepG2 cells were transfected with increasing amounts of ZHX2 and BEL7402 cells were transfected with increasing amounts of siZHX2, and the relative expression of Notch1 was quantified. (b) HepG2 and BEL7402 cells were co-transfected with pGL3- Notch1 along with pcDNA3.1 or increasing amounts of ZHX2, and the relative luciferase activity was measured, respectively. (c) Luciferase assay of the putative Notch1 promoter in HepG2 cells co-transfected with NF-YA or pcDNA3.1 or ZHX2. (d) ChIP analysis showed that ZHX2 repressed NF-YA occupying on the putative Notch1 promoter in HepG2 cells. All experiments were performed in triplicate. *P<0.05, **P<0.01