Figure 2 | Cell Death & Differentiation

Figure 2

From: TGFβ pathway limits dedifferentiation following WNT and MAPK pathway activation to suppress intestinal tumourigenesis

Figure 2

Loss of transforming growth factor-β (TGFβ) signalling does not affect the normal homeostatic proliferation. (a) Quantitative real-time-PCR (qRT-PCR) analysis of Tgfbr1 and (b) Tgfbr2 expression in wild-type freshly purified crypts (WT-Crypt) versus villi (WT-Villus). Data are shown as ratios to the internal Gapdh control with error bars representing mean±S.E.M., *P=0.04 by Mann–Whitney test, one-tailed, n=3 biological replicates. (c) RNAscope analysis of Tgfb1 in WT crypt and villus. Scale bars, 20 μm. (d) IHC analysis of pSMAD3 on WT small intestine. (e) Haematoxylin and eosin (H&E) and bromodeoxyuridine (BrdU) staining of WT, VilCreERTgfbr1fl/fl (Vil Tgfbr1fl/fl) and VilCreERSmad4fl/fl (Vil Smad4fl/fl) small intestine 4 days post induction with tamoxifen. Scale bars, 100 μm. (f) Quantification of total BrdU+ cells in WT, Vil Tgfbr1fl/fl and Vil Smad4fl/fl small intestine. Error bars represent mean±S.E.M., n=3 biological replicates

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