Figure 3
From: Cytotoxicity of apigenin on leukemia cell lines: implications for prevention and therapy
Analysis of cell proliferation pathways. Effect of apigenin treatment on key kinases involved in cell proliferation from HL60 (a) and TF1 (b) cells. (c) Effect of apigenin treatment on phosphatases involved in proliferation of HL60 and TF1 cells. Cells were treated with specified concentrations of apigenin for 24 h and the expression or phosphorylation of the proteins was determined using western blot analysis. Equal loading was confirmed by reprobing blots for β-actin. (d) The activity of immunoprecipitated LMWPTP was measured after apigenin treatment of HL60 and TF1 cells by Malachite green assay. (e) Effect of apigenin treatment on proteins involved in cell survival control. HL60 cells were treated with 50 μM apigenin for 5, 12 and 24 h; TF1 cells were treated with 100 μM apigenin for 18, 24 and 36 h; and the phosphorylation of the proteins was determined by western blotting. Equal loading was confirmed by reprobing them for β-actin
