Figure 4
From: Cytotoxicity of apigenin on leukemia cell lines: implications for prevention and therapy
Analysis of apigenin-induced apoptosis in HL60 and TF1 cells. (a) Percentage of apoptotic cells after 24 h treatment with apigenin (25, 50, and 100 μM) was determined using flow cytometric analysis of Annexin V staining. (b) The effect of pan-caspase inhibitor Z-VADfmk on apigenin-induced cell death. Cells were pretreated with 50 μM Z-VADfmk for 1 h before apigenin treatment. HL60 cells were treated with 50 μM apigenin and TF1 cells were treated with 100 μM apigenin for 24 h. (c and d) Effect of PI3K inhibitor Ly294002 on apoptosis induction in HL60 (c) and TF1 (d) cells. Cells were pretreated with 5 μM Ly294002 for 1 h before 24 h apigenin treatment. Afterwards, cells were stained with propidium iodide (PI) and Annexin V. The combined Annexin-V-positive fractions were considered apoptotic (***P<0.001, **<0.01)
