Figure 2
From: Imaging multiple phases of neurodegeneration: a novel approach to assessing cell death in vivo
Time-lapse video analysis showing changes in cell markers in vivo. (a–g) Stills taken from video sequence (Supplementary Video 1) in an SSP-treated rat eye with labelled RGCs (DiI, blue) and intravitreal annexin V (green) and propidium iodide (PI, red) reveal a random distribution and appearance of annexin V- and PI-stained cells, but demonstrate the clear increase in PI staining over time. (DI, blue; annexin V, green; propidium iodide, red; time points and scale bar 100 μm as indicated). (f, g) Average fluorescence intensity projections of video sequences using ImageJ RGB Zprojector plugin with annexin/PI (f) and all three channels (g). (h) Temporal analysis of the average annexin (green) and PI (red) intensities taken from each image sequence histogram demonstrates that the profiles of annexin and PI change significantly over time, with a marked increase in late PI staining
