Figure 6
From: Imaging multiple phases of neurodegeneration: a novel approach to assessing cell death in vivo
Retinal cell death in the Alzheimer Triple Transgenic model. (a, b) Representative wide-angle retinal images of a 14-month-old Alzheimer Triple Transgenic (3xTg-AD, a) mouse acquired 2 h after intravitreal PI (red) and annexin (green), compared with (b) an 18-month-old PS1KI control mouse. (c) Quantitation of co-localisation scatter plot data showing significantly more RGCs in the early phase of apoptosis (P<0.05, green, Anx+) and relatively fewer necrotic cells (P<0.05, red, PI+) in the 3xTg-AD model compared with control. (d, e) Retinal images of the eyes of a 14-month-old 3xTg-AD animal (d) and a control aged animal (e) acquired 2 h after PMA was administered at the same time as intravitreal annexin (green) and PI (red). (f, g) Quantification of the data generated by co-localisation scatter plot analysis showed that PMA treatment significantly increased early apoptosis (P<0.05, green, Anx+) and decreased late apoptosis (P<0.05, yellow, Anx+/PI+) in the 3xTg-AD model (g). PMA also increased the level of early apoptosis in aged control animals (P<0.05, green, Anx+), although not to the same extent (30.3% in aged control as opposed to 61.4% in 3xTg-AD). *P<0.05
