Figure 1 | Cell Death & Disease

Figure 1

From: Prions and neuronal death

Figure 1

Sheep. Tangential histological sections from an ileal myenteric plexus (MP) of a natural scrapie-affected Sarda breed sheep carrying the homozygous (scrapie-susceptible) ARQ/ARQ PrP genotype. Specific anti-nNOS (neuronal nitric oxide synthase) (a) and anti-PrPd (‘disease-specific PrP’ or ‘pathological PrP’) (b) immunolabelling are shown within neuronal perykaria and nerve fibres. Merging (c) and colocalization (d) of the two signals are shown in yellow-orange and in white colour, respectively, with a strong colocalization of nNOS and PrPd immunostaining being detected (d). Double-labelling indirect immunofluorescence with anti-nNOS (rabbit polyclonal) and anti-PrP (mouse monoclonal) commercial antibodies. Fluorescein avidin DCS (D cell sorter) (green colour) and Texas Red avidin DCS (red colour) were used as fluorochromes. Merging and colocalization analyses were carried out by means of ImageJ software. Bars=50 microns

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