Figure 2
From: p53-mediated delayed NF-κB activity enhances etoposide-induced cell death in medulloblastoma
MB cell lines displayed different sensitivity to etoposide involving both caspase-dependent and -independent cell death. (a) Cell viability was analysed by MTS assay at indicated time points, upon 20 μM etoposide treatment. (b) Viability of D283-MED cells treated with 20 μM etoposide in the presence of 5 μM Bay11-7082, 10 μM Wedelolactone or 5 μM JSH23 was assessed by MTS assay. (c) D283-Med cells were transfected with 100 nM of siRNA directed against p65 or a control scrambled siRNA. After 48 h, cells were treated with 20 μM etoposide for 8 h and viability was assessed by MTS assay. The knockdown efficiency was controlled by western blot (insert). (d) Several MB cell lines were treated with 20 μM etoposide for indicated times. Caspase 3/7 activity was measured with Caspase-Glo 3/7 assay kit. Results are expressed as fold-induction compared with control non-treated cells as a function of time. (e) D283-MED cells were treated with 20 μM etoposide in presence or absence of 5 μM Bay11-7082 for 8 h. Caspase 8 and 3/7 activities were assessed with Caspase-Glo assay kits. Results are relative to control non-treated cells. a–d results are the mean of three independent experiments±S.E.M. ‘***’ indicates statistical difference with P<0.001
