Figure 1
From: Protein kinase Cθ is required for cardiomyocyte survival and cardiac remodeling
PKCθ is expressed in cardiomyocytes and it is rapidly activated after pressure overload. (A) Immunolocalization of PKCθ in cryosections of LV from 2-month-old WT mice (a). Lack of PKCθ expression in PKCθ−/− LV is shown in b. The α-MyHC MF20 antibody (green) was used to identify cardiomyocytes (c, WT; d, PKCθ−/−). (Bar=50 μm). (B) Western blot analysis of PKC isoforms expression in LV from 2-month-old WT and PKCθ−/− mice. Representative experiment is shown (n=9 per genotype). The α-tubulin level of expression was used for normalization. (C) Left panel: representative western blot of the indicated PKC isoforms content in both cytosolic (Cy) and particulate (P) subcellular protein fractions, after 15 min transverse aortic constriction (TAC). Sham-operated mice (Sham) were used as controls. Right panel: densitometric analysis of the results obtained from western blots of the indicated PKC isoforms content in subcellular protein fractions (empty portion of each column=particulate fraction, P; gray portion=cytosolic fraction, Cy) expressed as relative percentage, with 1.0 given as the total level of expression of each isoform. The results were obtained as the mean value (±S.D.) of western blots prepared from protein subcellular fractions of single animals (n=8 per genotype: 4 sham operated, S, and 4 subjected to TAC, T). (D) Western blot analysis of the phosphorylated (active) form of PKCθ (Thr538p-PKCθ) in total protein fractions, after 15 min TAC. The phospho/total PKCθ ratio was evaluated by densitometric analysis and shown in the right. S=sham; T=TAC. *P<0.01
