Figure 2
From: Protein kinase Cθ is required for cardiomyocyte survival and cardiac remodeling
PKCθ ablation results in increased fibrosis. (A) Gomori's trichrome-stained representative cryosections of LV from 2-month-old WT (a and c) and PKCθ−/− mice (b and d); a–b: bar=2 mm; c–d: bar=200 μm. (B) RT-PCR analysis of the indicated fibrotic markers in LVs from 2-month-old WT and PKCθ−/− mice (3 mice per genotype). Col1α1: α1 collagen 1; Col3α1: α1 collagen 3; αSMA: α-smooth muscle actin; TGFβ1: transforming growth factor β1; GAPDH expression was used to normalize the reaction. (C) Quantification of interstitial fibrosis was examined at the indicated postnatal ages of WT (empty bars) and PKCθ−/− (filled bars) LVs, expressed as a percentage of the total area of the entire section. At least three sections per mouse were analyzed (n=3 per genotype/age). *P<0.01; **P<0.001; ***P<0.0001
