Figure 3
From: Protein kinase Cθ is required for cardiomyocyte survival and cardiac remodeling
PKCθ ablation results in increased fibroblast proliferation. (A) BrdU incorporation (green) in 2-month-old WT (a–c) and PKCθ−/− (d–f) LVs. Sections were counterstained with Hoechst and α-MyHC antibody (red) (b–c and e–f). Representative micrograph of transverse sections at higher magnification is shown in c and f (bar=50 μm). The percentage of BrdU+ve nuclei per section over the total number of nuclei is also shown (P<0.01 versus WT; n=3 per genotype). RT-PCR analysis of PCNA and Cyclin D1 expression in 2-month-old WT and PKCθ−/− LVs is shown in the right. GAPDH expression was used to normalize the reaction. (B) Immunofluorescence analysis of BrdU incorporation in cultured CFs isolated from LV of 1- to 3-day-old WT and PKCθ−/− mice (bar=50 μm). The percentage of BrdU+ve nuclei per microscopic field over the total number of nuclei is also shown (P<0.01 versus WT). RT-PCR analysis of Coll 1α1 expression in WT and PKCθ−/− cultured CFs is shown in the right. GAPDH expression was used to normalize the reaction
