Figure 4
From: Phosphorylation of Puma modulates its apoptotic function by regulating protein stability

Mutation of Puma serine 10 does not affect association of Puma with Mcl-1, Bcl-2 or Bcl-xL. Cells expressing Myc-tagged Puma-WT or Puma-S10A were immunoprecipitated with anti-Myc antibody and precipitated proteins were analysed by western blotting with antibodies against: Mcl-1, Bcl-2, Bcl-Xl and Puma