Figure 6
From: Novel p63 target genes involved in paracrine signaling and keratinocyte differentiation
Loss of TCF7L1 results in early differentiation of NHEKs in OTC. (a) NHEKs were transfected with siRNAs targeting TCF7L1 or non-silencing oligos (NS) as a control. At 48 h after transfection, RNA was isolated and qRT-PCR performed to assess the expression of TCF7L1. (b) Immunofluorescent analysis of control and siTCF7L1 OTCs were performed with antibodies specific for filaggrin, involucrin, loricrin, keratin 2, and E-cadherin. Dotted line represents the epidermis–dermis interface. For proliferation analysis, OTCs were grown in medium containing BrdU for 16 h before harvesting in order to label proliferative cells. Immunofluorescence with a BrdU-specific antibody was performed to identify actively proliferating cells in the epidermal layers of OTCs. (c) BrdU-positive cells from (b) were quantified and graphed. Data shown are representative of three independent experiments
