Figure 2 | Cell Death & Disease

Figure 2

From: Androgens modulate autophagy and cell death via regulation of the endoplasmic reticulum chaperone glucose-regulated protein 78/BiP in prostate cancer cells

Figure 2

Androgen suppresses autophagy induction independently of mTOR signalling. (a) Cells that were maintained for 24 h in SF medium alone or with 10 ng/ml mibolerone (SF+mib), 1 μM rapamycin (SF+rapa) or in combination (SF+mib+rapa) were lysed and western blotted with the indicated antibodies, with actin as a loading control. All panels are representative of at least three independent experiments. (b) Graphs depict densitometric analysis of band intensities of p-mTOR, p-S6K, p-S6 and p-4E-BP1, normalized to total protein levels and expressed as fold change from untreated control. (c) Representative confocal images of cells transiently expressing GFP-LC3 maintained for 24 h in the indicated conditions. Scale bar=20 nm. (d) Quantification of GFP-LC3 punctae-positive cells described in (c). A positive cell contained 10 or more punctae. n=3, 50 cells per condition per experiment, For (b) and (d), error bars indicate mean S.E. In (b), **P<0.01 versus FBS; in (d), **P<0.01 as indicated; and #P<0.01 versus FBS

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