Figure 3 | Cell Death & Disease

Figure 3

From: Androgens modulate autophagy and cell death via regulation of the endoplasmic reticulum chaperone glucose-regulated protein 78/BiP in prostate cancer cells

Figure 3

Effect of androgen on serum starvation-induced ER stress. For all panels, cells were maintained in FBS, SF or SF+mib for the indicated time points. For (a), (c) and (e), cells were lysed and western blotted with the indicated antibodies. Graphs depict densitometric analysis of band intensities of indicated proteins, normalized to total protein levels and expressed as fold change from untreated control. For (b), (d) and (f), total RNA was extracted and mRNA was quantified by RT-PCR and normalized to actin levels (b and d) or subjected to 2% agarose gel separation (f). (a and b) Expression of Grp78/BiP protein and mRNA. (c) Activation of eIF2α and CHOP. (d) Levels of ATF4 mRNA. (e) ATF6 protein expression and cleavage. Full-length (90 kDa) and cleaved (50 kDa) forms of ATF6 are indicated by arrows. n/s=non-specific band. (f) XBP-1 mRNA splicing. Full length and spliced XBP-1 are indicated by arrows. Cells treated for 6 h with 2 μM thapsigargin (TG) were used as a positive control. All panels are representative of at least three independent experiments. Error bars indicate mean S.E., *P<0.05, **P<0.01 versus FBS or as indicated. For (d), *P<0.05 SF+mib versus all groups

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