Figure 4 | Cell Death & Disease

Figure 4

From: Androgens modulate autophagy and cell death via regulation of the endoplasmic reticulum chaperone glucose-regulated protein 78/BiP in prostate cancer cells

Figure 4

Knockdown of Grp78/BiP prevents androgen-mediated inhibition of autophagy. (a) Control siRNA- and Grp78/BiP-siRNA-treated cells were maintained for 24 h in FBS, SF and SF+mib before lysis. Lysates were analysed by western blot, with GAPDH as loading control. (b) Graphs depict densitometric analysis of band intensities of indicated proteins, normalized to total protein levels and expressed as fold change from untreated control. (c) Representative confocal images of cells stably expressing mRFP-GFP-LC3 treated as described in (a). Merged images depict overlay of GFP (green) and mRFP (red) images. Scale bar=20 nm. (d) Quantification of mRFP+ (right panel) and mRFP+/GFP+ (left panel) punctae in cells shown in (c). n=3, 25 cells per condition per experiment. For (b) and (d), error bars indicate mean S.E., *P<0.05 and **P<0.01 versus FBS or as indicated

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