Figure 5 | Cell Death & Disease

Figure 5

From: Androgens modulate autophagy and cell death via regulation of the endoplasmic reticulum chaperone glucose-regulated protein 78/BiP in prostate cancer cells

Figure 5

Blocking eIF2α dephosphorylation with salubrinal does not impair androgen-mediated autophagy inhibition. (a) Cells treated for 24 h in FBS, SF or SF+mib with 50 μM salubrinal or DMSO vehicle control were lysed and western blotted with the indicated antibodies. Actin was used as loading control. (b) Graphs depict densitometric analysis of band intensities of indicated proteins, normalized to total protein levels and expressed as fold change from untreated control. (c) Representative confocal images of cells treated as described in (a). Scale bar=20 nm. (d) Quantification of GFP-LC3 puncta-positive cells shown in (c). A positive cell contained five or more punctae. n=3, 50 cells per condition per experiment, For (b) and (d), error bars indicate mean S.E., *P<0.05 and **P<0.01 as indicated; #P<0.01 versus FBS

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