Figure 6 | Cell Death & Disease

Figure 6

From: Androgens modulate autophagy and cell death via regulation of the endoplasmic reticulum chaperone glucose-regulated protein 78/BiP in prostate cancer cells

Figure 6

Androgen delays chronic serum starvation-induced autophagy. (a and b). Time course of autophagy induction and trafficking in mRFP-GFP-LC3 stable cells. Quantification of mRFP+/GFP+ (a) and mRFP+ (b) punctae in cells maintained in SF or SF+mib for 24, 48, 72 and 98 h. Twenty-five cells were analysed per condition per experiment, n=3, error bars indicate mean S.E., *P<0.05. (c). Representative confocal images of cells maintained in FBS medium or after 96 h of SF or SF+mib. Merged images depict overlay of GFP (green) and mRFP (red) images. Scale bar=20 nm. (d) Cells were treated as described in (a), lysed and subjected to western blot analysis with the indicated antibodies. All panels are representative of three independent experiments

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