Figure 1
From: Inhibition of NF-κB activation sensitizes U937 cells to 3′-azido-3′-deoxythymidine induced apoptosis
Effect of AZT on apoptosis in U937 cell. (a) U937 cells were treated with 0, 8, 32, 128 μM AZT for 3, 6, 18, 24 and 48 h and apoptosis was evaluated by hypodiploid nuclei analysis after DNA staining with PI. Apoptosis was evaluated by flow cytometry analysis of hypodiploid nuclei and expressed as mean values ± S.E. obtained from three independent experiments; asterisk indicates a significant difference versus CTR (*P<0.05; **P<0.010). (b) FACS analysis of annexin-V and PI in U937 cells treated with vehicle or 128 μM AZT, after 18 h in culture. (c) Percentage of hypodiploid nuclei in U937 cells treated with vehicle or 128 μM AZT, after 48 h in culture. In Figure 1b and c one representative out of the three independent experiments performed is shown
