Figure 1
From: Caspase cleavage of the Golgi stacking factor GRASP65 is required for Fas/CD95-mediated apoptosis
Expression of caspase-resistant GRASP65 protects cells from Fas-mediated apoptosis. (a) Fluorescence images of GRASP65 in standard Hela cells (labelled with GRASP65 antibodies) and HeLa cells stably expressing wild-type or caspase-resistant GRASP65–GFP. Bar=20 μm. (b) Apoptosis responses of standard HeLa cells, and HeLa cells stably expressing wild-type or caspase-resistant GRASP65–GFP to various apoptotic stimuli. Apoptotic cells were scored by immunofluorescence detection of caspase-cleaved PARP. Data show means±S.D. for a minimum of three experiments. Students’ t-test: ***P<0.001, NS, not significant. (c) Time-matched frames from time lapse movies of HeLa cells stably expressing wild-type and caspase-resistant GRASP65–GFP treated with Fas ligand, comparing the rates of apoptosis (rounded, blebbing profiles) between these cell lines (frames correspond to Supplementary Video 1). Bar=20 μm
