Figure 3
From: Caspase cleavage of the Golgi stacking factor GRASP65 is required for Fas/CD95-mediated apoptosis
Fas/CD95 trafficking and Golgi architecture in GRASP65 cell lines. (a) Immunoblot of cleaved PARP showing the influence of BFA and nocodazole treatment on apoptosis in GRASP65 cell lines treated with Fas ligand (FasL; 6 h). (b) Immunoblot of total and surface Fas/CD95 following biotinylation enrichment from the GRASP65 expressing cell lines shown. <, Surface Fas/CD95. (c) Fluorescence recovery after photobleaching (FRAP) analysis of Golgi structural integrity in wild-type and N320 GRASP65–mCherry cells. The trace shows the recovery of NAGFP fluorescence (%) into the bleached region (hatched box in the example images). Data are means ± S.D. for wild-type GRASP65–GFP and N320 GRASP65–mCherry of three cells and six cells, respectively
