Table 1 Kinetic parameters of trimer–monomer complex using surface plasmon resonance

From: Rapid and efficient cancer cell killing mediated by high-affinity death receptor homotrimerizing TRAIL variants

 

DR4-Ig

Protein

k a (/M/s) a

kd (s−1)b

K D (nM) c

WT

(7.45±0.4) × 105

(1.66±0.3) × 10−4

0.22±0.06

4C7

(28.1±1.7) × 105

(0.59±0.3) × 10−4

0.021±0.01

4C9

(23.5±1.4) × 105

(0.58±0.4) × 10−4

0.025±0.02

 

DR5-Ig

WT

(13.9±1.5) × 105

(0.65±0.3) × 10−4

0.033±0.03

4C7

(1.14±0.5) × 105

(8.48±0.8) × 10−4

7.41±4.2d

4C9

(19.0±0.7) × 105

(23.1±1.2) × 10−4

1.22±0.2d

  1. Association rate constant.
  2. bDissociation rate constant.
  3. cEquilibrium dissociation constant (kd/ka). Each value was calculated using the BIAcore 3000 and the BIA evaluation 4.1 software.
  4. dCurves showed heterogeneity, a phenomenon that may be correlated to partial glycosylation of receptors
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