Figure 1 | Cell Death & Disease

Figure 1

From: Synergistic induction of cell death in liver tumor cells by TRAIL and chemotherapeutic drugs via the BH3-only proteins Bim and Bid

Figure 1

Synergistic induction of cell death by doxorubicin and TRAIL. (a) HepG2, Huh7, Hep3B and IHH cells were pretreated by either medium control, 10 ng/ml TRAIL (left panels) or 1 μg/ml doxorubicin (Dox, right panels), before the exposure to increasing concentration of doxorubicin (left panels) or TRAIL (right panels). Cell death was measured by MTT assay. Mean values±S.D. of quadruplicates of a typical experiment (n=3) are shown. (b) HepG2 cells were treated with 1 μg/ml doxorubicin, 10 ng/ml TRAIL or the combination thereof for 6 h, and DEVDase activity in cell lysates was measured. Mean values±S.D. of quadruplicates were measured. n=3. (c) HepG2 cells were pretreated with medium control or 10 ng/ml TRAIL, and then exposed to increasing doses of doxorubicin. Apoptosis was measured by Annexin V binding. n=2

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