Figure 2

Effects of pretreatment on synergistic induction of cell death. HepG2 cells were pretreated with medium control, TRAIL (left panel, 10 ng/ml) or doxorubicin (Dox, right panel, 1 μg/ml), either 60 min before adding increasing doses of doxorubicin or TRAIL, or directly together with the apoptosis trigger (0 min). Cell death was measured by MTT assay. Mean values±S.D. of quadruplicates are shown. n=2