Figure 5

TRAIL promotes Bim mobilization and phosphorylation. (a) HepG 2 cells were treated for indicated time with 1 μg/ml doxorubicin (Dox), 10 ng/ml TRAIL, or the combination thereof. Bim levels in NP40 lysates were analyzed by western blot. Detection of JNK was used for loading control. Positions of Bim and phosphorylated Bim (P-Bim) are shown. (b) Cells were treated as indicated above for 4 h, lyzed in SDS buffer and total Bim and JNK levels were analyzed by western blot