Figure 7

Role of Bid and Bim in TRAIL plus doxorubicin (Dox)-induced cell death. (a) HepG2 cells were treated with doxorubicin, TRAIL or both for indicated time intervals and Bid cleavage was analyzed by western blot. (b) HepG2 cells were transfected with control (Contr.) siRNA, Bim or Bid specific siRNA or the combination of both. Downregulation of Bim and Bid was analyzed by western blot. Tubulin was used as loading control. (c) Cells were treated with siRNA as indicated above and apoptosis sensitivity to doxorubicin (1 μg/ml), TRAIL (10 ng/ml) or both was analyzed by DEVDase clevage assay. Mean values±S.D. of quadruplicates are shown. n=3. ^*P<0.005, ^**P<0.0005 compared with control siRNA. tBid, truncated Bid