Figure 6 | Cell Death & Disease

Figure 6

From: The CD14+/lowCD16+ monocyte subset is more susceptible to spontaneous and oxidant-induced apoptosis than the CD14+CD16− subset

Figure 6

CD16+ monocytes exhibit higher aerobic respiration. (a) Histogram plots showing the intracellular levels of reactive oxygen radicals as measured by DCFH for general ROS, by DHE for superoxide anions and by APF for hydroxyl and hypochlorite radicals in unstimulated lymphocytes (solid line), CD16− (dotted line) and CD16+ (dash line) monocytes. (b) Western blot analysis of ATP5B and NDUFS3 from isolated monocyte subsets from three different individuals to validate the proteomics data. The numbers below the blots are relative intensity values after normalizing with the actin control. (c) Bar graph depicts the intracellular ATP levels in isolated monocyte subsets. (d) FACS analysis using MitoSox to assess mitochondrial superoxide from either freshly isolated monocyte subsets (0 min) or after in vitro culture for 30 min. The numbers represent percentages of cells within each box that had intracellular mitochondrial superoxide with respect to total cells. All data shown are from at least three independent experiments and error bars represent S.E.M. ** indicates P<0.01

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