Figure 2 | Cell Death & Disease

Figure 2

From: Downregulation of microRNA-383 is associated with male infertility and promotes testicular embryonal carcinoma cell proliferation by targeting IRF1

Figure 2

miR-383 functions as a negative regulator of proliferation in NT2 cells. (a and d) miR-383 repressed cell proliferation. Cell viability was measured by the cell counting kit-8 assay after transfection with 25, 50 or 100 nM mimic negative control (Mimic NC) or miR-383 mimic (panel a) or with 150 nM inhibitor negative control (Inhibitor NC) or miR-383 inhibitor (panel d). (b and e) miR-383 induced G1-phase arrest. Cell-cycle profiles were analyzed using flow cytometry on cells transfected either with 150 nM miR-383 mimic (panel b) or with 150 nM miR-383 inhibitor (panel e). (c and f) miR-383 promoted cell apoptosis. The apoptosis rate was measured using flow cytometry with Annexin V and PI staining after transfection either with 100 nM miR-383 mimic (panel c), or with 150 nM miR-383 inhibitor (panel f). Data are presented as means±S.E.M. (n=3). *P<0.05, **P<0.01, compared with negative controls

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