Table 2 Expression of the indicated proteins or miR-383 in human testicular specimens

From: Downregulation of microRNA-383 is associated with male infertility and promotes testicular embryonal carcinoma cell proliferation by targeting IRF1

  

Testicular tissue (ages)

Gene

Cell type

N1 (28)

N2 (69)

A1 (30)

A2 (26)

A3 (35)

A4 (20)

A5 (27)

A6 (27)

A7 (34)

A8 (28)

A9 (24)

A10 (27)

miR-383

—

+

ND

−

−

−

−

−

−

ND

ND

ND

+

PCNA

Spermatogonia

+/−

ND

+/−

+

+

+

+/−

+

+/−

ND

NE

ND

 

Spermatocyte

+/−

 

+/−

+

+

+

+/−

+

+/−

   
 

Spermatid

+/−

 

NE

NE

NE

NE

NE

NE

NE

   
 

Sertoli cell

−

 

−

−

−

−

−

−

−

 

−

 

IRF1

Spermatogonia

−

+/−

+

−

−

ND

+/−

+

+/−

+/−

ND

 
 

Spermatocyte

−

+/−

+

−

+

 

+/−

+

−

+/−

  

Cyclin D1

Spermatogonia

−

ND

−

+/−

ND

 

+

+

 

ND

  
 

Spermatocyte

−

 

−

−

  

−

+

    

p21

Spermatogonia

−

 

−

−

−

 

−

+/−

    
 

Spermatocyte

−

 

−

−

−

 

−

+/−

    

CDK2

Spermatogonia

−

 

−

−

ND

 

+/−

+

    
 

Spermatocyte

−

 

−

−

  

+/−

+

    

CDK4

Spermatogonia

+/−

 

+

+

  

+

+

    
 

Spermatocyte

+/−

 

+

+

  

+

+

    

P-pRb

Spermatogonia

+/−

 

+

+

+/−

+

+

+

    
 

Spermatocyte

+/−

 

+

+

+/−

+

+

+

    
  1. Expressions of the indicated proteins were determined by immunohistochemical analysis. Levels of mature miR-383 were examined by real-time PCR and/or in situ hybridization. For immunohistochemistry, the presence of nuclear staining for the indicated proteins was regarded as a positive expression, whereas cytoplasmic staining was regarded as a negative expression. Data are presented as three categories: (−), no detectable staining or positive staining of up to 5%; (+/−), positive staining of 5–40%; (+), positive staining of 40–90% cells. A1–A8, maturation arrest; A9, Sertoli-cell-only syndrome; A10, hypospermatogenesis; N1–N2, normal control; ND, not detected; NE, not existing
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