Figure 5
From: The acetylation of RelA in Lys310 dictates the NF-κB-dependent response in post-ischemic injury
(a) Cell death was evaluated in cortical neurons exposed to 3 h OGD and then treated with 1, 3, 30 μM resveratrol for 24 h. Resveratrol produced a significant neuroprotective effect at the 30 μM concentration. Bars are the means±S.E.M. of three separate experiments run in quadruplicate; *P<0.05 versus the corresponding control value. (b) Representative picture of co-immunoprecipitation analysis of RelA in nuclear extracts of primary cortical neurons exposed to 3 h OGD and to 30 μM resveratrol for 2 h during the post-OGD period. (c) Luciferase activity in cortical neurons co-transfected with Bim luciferase reporter plasmid and control vector (pSG5) or RelA-K310R expression plasmid. OGD stimulated luciferase activity in pSG5 but not in RelA-K310R-expressing cells. Resveratrol (30 μM), added after the OGD and maintained for 4 h, repressed luciferase activity in pSG5-cells. Bars are the means±S.E.M. of three separate experiments; *P<0.01, **P<0.05 versus OGD value in pSG5-expressing neurons
