Figure 2

(a) Immunoblot analysis of extrinsic death pathway activation including cleavage of caspase 8, caspase 3, and PARP (arrows) in U251HF cells treated with AdsTRAIL or sTRAIL for the periods indicated. Soluble TRAIL (sT)-treated cells were assessed as control. (b) Comparison between the effects of AdsTRAIL (AdsT) or AdEGFP (AdE) upon caspase 8, caspase 3, and PARP in U251HF cells and NHAs. Cells were treated with 100 MOI of adenovirus and harvested at 24 h