Figure 5
Effects of AdsTRAIL on survival in an intracranial glioma xenograft model: (a) U251HF cells were implanted in the right forebrain of nude mice and tumor formation was determined by bioluminescence imaging; mice with confirmed tumors were equally distributed based on tumor sizes into treatment groups on day 0 and monitored for change in bioluminescent signal after treatment with PBS, AdEGFP, or AdsTRAIL over the period indicated. (b) Animals intratumorally injected with PBS, AdEGFP, or AdsTRAIL were monitored at regular intervals for the first 28 days by quantitative assessment of bioluminescence as a surrogate for tumor growth. (c) A subset of animals were killed at day 3 after adenoviral injection and paraffin-embedded sections generated from the tumor-bearing brains. Morphology was studied using H&E staining; immunohistochemical studies were used to examine cleaved caspase 3 and TUNEL positivity. Higher magnification of the H&E-stained tumor tissue is shown in the right panel. (d) U251HF cells were implanted in the right forebrain of nude mice and, after confirming tumor growth, intratumoral injection of PBS (dotted line), AdEGFP (dashed line), or AdsTRAIL (solid line) was performed and the animals monitored for survival. Probability of overall survival was determined by Kaplan–Meier analysis and the significance of differences assessed by log rank test
