Figure 3 | Cell Death & Disease

Figure 3

From: C6-ceramide synergistically potentiates the anti-tumor effects of histone deacetylase inhibitors via AKT dephosphorylation and α-tubulin hyperacetylation both in vitro and in vivo

Figure 3

Endogenous ceramide production is involved in TSA-induced cancer cell death. CaOV3 (a–c) or L3.6 (d–f) cells were pretreated with the glycosphingolipid biosynthesis inhibitor 1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP, 10 μM) or the acid sphingomyelinase inhibitor desipramine (DSP, 30 μM) for 2 h, followed by 200 ng/ml of TSA treatment, endogenous cellular ceramide production (indicated as fold change) was detected by the methods as described above after 12 or 48 h (a and d), cell death and apoptosis were detected by MTT assay (b and e, 48 h after TSA treatment) and Histone DNA ELISA assay (c and f, 36 h after TSA treatment) independently. Data are presented as the mean±S.D. for three independent experiments. Experiments in this figure were repeated at least three times and similar results were obtained. *P<0.05 versus that indicated in the figure (ANOVA)

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