Figure 7 | Cell Death & Disease

Figure 7

From: C6-ceramide synergistically potentiates the anti-tumor effects of histone deacetylase inhibitors via AKT dephosphorylation and α-tubulin hyperacetylation both in vitro and in vivo

Figure 7

C6-ceramide enhances the anti-tumoral efficacy of TSA in vivo. Mice survival and tumor growth were compared with L3.6 (n=10/group) (a and c) and CaOV3 (n=10/group) models (b and d). In both models, only groups that received both TSA plus C6-ceramide treatment showed significantly smaller tumor volumes and enhanced mice survival as compared with the mock treatment (control) group, and the group that received single treatment (TSA or C6-ceramide) showed very limited effect in tumor volume/mice survival. AKT activation and tubulin acetylation were analyzed. In both models, at 4 weeks after drug treatment (mock, TSA, C6-ceramide, combination), tumor xenograft were freshly removed, solubilized and lysed using lysis buffer, followed by western blot detecting AKT phosphorylation and tubulin acetylation (e and f). The effects of different treatments (mock, TSA, C6-ceramide, C6-ceramide plus TSA) on body weight of mice in the xenograft models were demonstrated in g (L3.6 cell model) and h (CaOV3 model). All experiments were repeated at least three times and similar results were obtained

Back to article page