Figure 3

GZMB is abundant in vessels exhibiting medial disruption. Serial sections of abdominal aorta were taken from a sham-treated mouse following aortic rupture and stained for Movat's Pentachrome (4 × : a, 40 × : b and c), GZMB (4 × : d, 40 × : e and f) and decorin (4 × : g, 40 × : h and i). GZMB staining by immunohistochemistry (d and f) corresponds to regions of medial disruption and elastin fragmentation (a and c) and loss of decorin in the adventitia (g and i). The non-dilated side of the aorta has reduced GZMB staining in the media and adventitia (d and e) and corresponds to intact elastic lamellae (a and b) and decorin (g and h), scale bars: 4 × , 500 μm; 40 × , 50 μm