Figure 5

Washing off WIN55,212-2 or treatment with CHX protect Granta519 from WIN55,212-2-induced cytoplasmic vacuolation and cell death. (a) Cell viability of Granta519 and Rec1 cells incubated with 10 μM WIN55,212-2 for 3 and 6 h before washing off the drug. Then cells were incubated for 48 h, and cell viability was measured by XTT (P<0.05). WB of Granta519 shows decreased LC3 lipidation by washing off 10 μM WIN55,212-2 after 6 h. (b) Cell viability (XTT) of Granta519 cells incubated with 10 μM WIN55,212-2 for 24 h, followed by washing off the drug and reculturing for 24 h (P<0.05). (c) CHX protects from WIN55,212-2-induced cell death measured by XTT; ^*indicates statistical significance (P<0.05)