Figure 1 | Cell Death & Disease

Figure 1

From: TNF-induced necroptosis in L929 cells is tightly regulated by multiple TNFR1 complex I and II members

Figure 1

Knockdown of HOIL-1, HOIP or A20 sensitizes to TNF-induced necroptosis, whereas depletion of TRADD or NEMO does not affect the cell viability. (a) Upon knockdown of RIP1, L929 cells were stimulated with TNF for the indicated times. IκBα degradation and RIP1 knockdown efficiency was analyzed using western blotting. Asterisk indicates an nonspecific band. Flow cytometric analysis of the percentage of Sytox-positive cells after TNF stimulation for 4 h (reaching around 50% cell death in control setup) of L929 cells in which TRADD or NEMO (b), HOIL-1 or HOIP (d), or A20 was knocked down (f). Error bars indicate S.D.s of triplicates of three independent experiments. Cell survival analysis using a MTT assay after 20 h of TNF stimulation of L929 cells in which TRADD or NEMO (c), HOIL-1 or HOIP (e), or A20 was knocked down (g). Error bars indicate S.Ds of triplicates. Knockdown efficiency of the indicated proteins was determined by western blotting or RT-PCR. *P<0.05 (Mann–Whitney U-test)

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