Figure 4 | Cell Death & Disease

Figure 4

From: TNF-induced necroptosis in L929 cells is tightly regulated by multiple TNFR1 complex I and II members

Figure 4

RIP3-dependent necroptosis when RIP1 and caspase-8 or TRADD are knocked down. (a) Flow cytometric analysis of the percentage of caspase-3 activity and PI-positive cells at the indicated time points after TNF stimulation of L929pCasper3-BG (FRET-based apoptosis sensor) cells in which RIP1, RIP1+caspase-8, or RIP1+caspase-8+RIP3 are knocked down. (b) Flow cytometric analysis of the percentage of caspase-3 activity and PI-positive cells at the indicated time points after TNF stimulation of L929pCasper3-BG cells in which RIP1, RIP1+TRADD and RIP1+TRADD+RIP3 are knocked down. Error bars indicate S.Ds of triplicates and data are representative of at least two independent experiments. Knockdown efficiency of the indicated proteins was determined by western blotting

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