Figure 1
From: A new vicious cycle involving glutamate excitotoxicity, oxidative stress and mitochondrial dynamics
OPA1 mutation reduces OPA1 protein expression and induces apoptotic cell death in the retina of Opa1enu/+ mice. (a) The OPA1 antibody recognized 90-kDa (L) and 80-kDa (S) isoforms of OPA1 protein in the total retinal protein extracts of wild-type control and Opa1enu/+ mice. Opa1enu/+ mice significantly decreased 80-kDa (S) isoform of OPA1 protein compared with wild-type control mice. Values are mean±S.D. (n=4 retinas/group). *Significant at P<0.05 compared with wild-type control mice. (b and c) OPA1 immunohistochemistry. (b) OPA1 immunoreactivity was present in the GCL of wild-type control mouse (arrowheads). In contrast, OPA1 immunoreactivity was decreased in the GCL of Opa1enu/+ mouse (arrowheads, c). (d–f) OPA1 (d) and FluoroGold (e) double labeling. Neurons containing OPA1 immunoractivity were colabeled by FluoroGold (arrowheads), indicating that RGCs contained OPA1 protein. There were no TUNEL-positive cells in the retina of wild-type control mouse (g) while apoptotic cell death was detectable in the GCL of the retina of Opa1enu/+ mouse (arrow, h). ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bars, 20 μm (b–e)
