Figure 5
From: A new vicious cycle involving glutamate excitotoxicity, oxidative stress and mitochondrial dynamics
OPA1 mutation decreases SOD2 gene and protein expression in Opa1enu/+ mice. (a) Opa1enu/+ mice significantly decreased SOD2 mRNA expression compared with wild-type control mice. (b) Opa1enu/+ mice significantly decreased SOD2 protein expression compared with wild-type control mice. Values are mean±S.D. (n=4 retinas/group). *Significant at P<0.05 compared with wild-type control mice. (c–e) SOD2 immunohistochemistry (green). When the primary antibody for SOD2 was omitted, there was no binding of the secondary antibody (c). Compared with wild-type control mouse (d), Opa1enu/+ mouse showed decreased SOD2 immunoreactivity in the OPL, IPL and GCL (e). ONL, outer nuclear layer; OPL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar, 20 μm (b–d)
