Figure 1 | Cell Death & Disease

Figure 1

From: Adaptation of cancer cells from different entities to the MDM2 inhibitor nutlin-3 results in the emergence of p53-mutated multi-drug-resistant cancer cells

Figure 1

Radiation sensitivity of the UKF-NB-3 cell line, its sublines resistant to nutlin-3 (UKF-NB-3rNutlin10 μM), VCR (UKF-NB-3rVCR10), CDDP (UKF-NB-3rCDDP1000), or DOX (UKF-NB-3rDOX20), and UKF-NB-3 cells transfected with lentiviral vectors expressing shRNA directed against p53 (UKF-NB-3p53−shRNA) or scrambled shRNA (UKF-NB-3scr−shRNA). A total of 1 × 104 cells were seeded per well in 96-well plates, allowed to adhere for about 5 h, irradiated, and viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay 24 h post irradiation. *P<0.05 relative to UKF-NB-3

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