Figure 5
From: Prevention of neonatal oxygen-induced brain damage by reduction of intrinsic apoptosis
TRP601 restores Bcl-2 expression under hyperoxic conditions. (a) Quantitative analysis of mRNA expression by real-time PCR showed a marked reduction of Bcl-2 mRNA expression in thalamic samples of rat pups that were kept for 12 and 24 h under hyperoxia (black bars), whereas TRP601 treatment restores Bcl-2 expression to control levels (gray bars). Application of TRP601 under room air (control, dashed bar) showed no significant regulation on Bcl-2 mRNA expression. (b) The analysis of Bcl-2 protein expression by western blot showed a similar expression pattern. The protein expression of Bcl-2 is significantly decreased after 12 and 24 h; the single application of TRP601 could restore the Bcl-2 protein expression almost up to control level. The densitometric data represent the ratio of the density of the Bcl-2 band to the corresponding β-actin band. Data are normalized to levels of rat pups exposed to normoxia (control 100%; bars represent mean±S.E.M., thalamus, n=6–8/group, ***P<0.001, ###P<0.001, one-way ANOVA compared with respective controls). Blots are representative of a series of three blots
