Figure 1 | Cell Death & Disease

Figure 1

From: DNA damage induces reactive oxygen species generation through the H2AX-Nox1/Rac1 pathway

Figure 1

Exposure to DNA-damaging agents leads to increased levels of ROS that contribute to cell death. (a) Sub-confluent U2OS and HBL100 cells were treated with the DNA-damaging agents neocarzinostatin (NCS, 0.5 μg/ml), hydroxyurea (HU, 2 mM), and doxorubicin (8.5 μM) for 20 min to 5 h. ROS levels were quantified using DCFH-DA and a fluorescent microplate reader. (b) Addition of 6 or 30 mM NAC 2 h after NCS treatment suppressed the latent NCS-induced increase in ROS. (c) Suppression of the latent NCS-induced ROS by NAC treatment reduced NCS-induced apoptosis

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