Figure 2
From: DNA damage induces reactive oxygen species generation through the H2AX-Nox1/Rac1 pathway
Upregulation and phosphorylation of H2AX is an early event in the DDR cascade. U2OS and HBL100 cells were treated with NCS (0.5 μg/ml), for 20 min to 5 h in PBS buffer (to mimic the condition of cells used for ROS assays). Levels and phosphorylation of H2AX and ATM protein were assayed by immunoblot analysis using antibodies indicated
