Figure 3
From: DNA damage induces reactive oxygen species generation through the H2AX-Nox1/Rac1 pathway
Histone H2AX increases basal and DNA damage-induced ROS. Overexpression of Flag-H2AX increased basal and NCS-induced ROS in U2OS (a) and HBL-100 (b) cells, whereas inducible H2AX knockdown reduces NCS-induced ROS in HCT-116 (c) and HBL100 (d) cells. Immunoblot analysis was performed at 5 h after NCS treatment. **Flag-tagged H2AX, *endogenous H2AX
