Figure 7
From: ERK2 phosphorylation of serine 77 regulates Bmf pro-apoptotic activity
Phosphorylation of serine 74 and serine 77 does not alter Bmf association with pro-survival proteins. HA-Bmf variants (WT, S74A, S77A and AA) were co-expressed in 293 FT cells with Myc-tagged B-RAFV600E and (a) Bcl-xL, (b) Bcl-2 or (c) Mcl-1 for 24 h. Immunoprecipitation was performed using antibody against the HA epitope tag. The presence of Bcl-xL, Bcl-2 and Mcl-1 in immunoprecipitates (HA IP) and levels of transgene expression in cell lysates (input) was examined by western blot analysis
