Figure 1
From: Consumption of oxygen: a mitochondrial-generated progression signal of advanced cancer
Reduction of the mitochondrial genome content in PCa and BCa reversibly induced aberrant activation of ERK and AKT. (a) Mitochondrial genome content per cell in low (6–7) and high (8–9) Gleason grade PCa separated by laser microdissection. Student's t-test compared PCa with a high Gleason grade (*P<0.01) (n=8 and 6, respectively). (b) Mitochondrial genome contents of single cells (n=10) of an early-stage (LNCaP) PCa cell line, and an array of (C4-2, PC-3 and DU-145) PCa cell lines. (c) FISH of the mitochondrial genome. Green: mitochondrial genome. Red: mitochondria. Scale bar represents 20 μm. (d) Mitochondrial genome content in single cells (n=8) of the parental (LNCaP and MCF-7), mtDNA-KO (LNρ0-8 and MCFρ0) and mtDNA-KI (LNCyb and MCFCyb) cells. (e) Phospho-ERK, total ERK, phospho-Akt, total AKT and β-actin in total-protein lysates (30 μg) of the indicated cell lines
